WormBase Tree Display for Expr_pattern: Expr1621
expand all nodes | collapse all nodes | view schema
| Expr1621 | Expression_of | Gene | WBGene00006808 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006808 | |||
| Expression_data | Life_stage | WBls:0000002 | ||
| Anatomy_term | WBbt:0004757 | Certain | ||
| WBbt:0004758 | Certain | |||
| WBbt:0004947 | Certain | |||
| WBbt:0004949 | Certain | |||
| WBbt:0005759 | Certain | |||
| WBbt:0005829 | Certain | |||
| WBbt:0006749 | Certain | |||
| WBbt:0006750 | Certain | |||
| GO_term | GO:0030424 | |||
| Subcellular_localization | UNC-76 protein was observed throughout the axons of wild-type animals, including some nonfasciculated axon segments known to be normal in unc-76 mutant animals (all motor neuron commissures and the ventral-directed segments of the HSN, PDE, and PVD axons). | |||
| Type | Antibody | Rabbit polyclonal antiserum. | ||
| Pattern | Intense staining showed in all major nerve bundles (the nerve ring and dorsal and ventral nerve cords) and all minor longitudinal and circumferential process tracts and bundles of sensory processes. Staining was visible in neuronal cell bodies but not in nuclei. Outside the pharynx, all neurons, but no other cells, stained with anti-UNC-76 antisera. Specifically, no staining was observed in cells that flank the ventral or dorsal nerve cords, such as body wall muscles or hypodermal cells. | |||
| UNC-76 staining was visible throughout the nervous system of animals at all developmental stages from newly hatched larvae through adults. Embryos were not sufficiently permeabilized to allow anti-UNC-76 antibody staining, but unc-76::lacZ fusions that appeared to be expressed normally in larvae and adults were expressed in a few cells, the identities of which were not determined, in embryos of about 200 cells, before the outgrowth of the first axons. UNC-76 staining in axons was strong throughout development, whereas cell body staining was strong in young larvae, but weaker in adults except for cell bodies in the head and tail ganglia. Of neurons with laterally positioned cell bodies, only the CAN and HSN cell bodies consistently contained UNC-76 protein in adults. The presence of C terminally truncated UNC-76 proteins in axons of mutant worms suggested that axon-targeting activity of UNC-76 resides in the N-terminal third of the protein. | ||||
| Picture | WBPicture0000011632 | |||
| Remark | No neuronal fluorescence was observed in worms stained with any of the three preimmune sera, and staining was reduced or nearly eliminated in all eight mutant unc-76 strains. | |||
| Pharyngeal UNC-76 expression was not visible, but pharyngeal cells were probably not made accessible to antibodies during fixation because they lacked the background stain seen in the rest of the animal. | ||||
| Reference | WBPaper00002751 | |||
| Antibody_info | WBAntibody00000143 |
