WormBase Tree Display for Expr_pattern: Expr16258

Expr16258 Expression_of: Gene: WBGene00003102
    Reflects_endogenous_expression_of: WBGene00003102
  Expression_data: Anatomy_term: WBbt:0003843 Certain
      WBbt:0003905 Certain
      WBbt:0003906 Certain
      WBbt:0003907 Certain
      WBbt:0003908 Certain
      WBbt:0003909 Certain
      WBbt:0003910 Certain
      WBbt:0003911 Certain
      WBbt:0004096 Certain
      WBbt:0004587 Certain
      WBbt:0004589 Certain
      WBbt:0004591 Certain
      WBbt:0004593 Certain
      WBbt:0004597 Certain
      WBbt:0004598 Certain
      WBbt:0004599 Certain
      WBbt:0004601 Certain
      WBbt:0004603 Certain
      WBbt:0004605 Certain
      WBbt:0004607 Certain
      WBbt:0004611 Certain
      WBbt:0004613 Certain
      WBbt:0004617 Certain
      WBbt:0004618 Certain
      WBbt:0004623 Certain
      WBbt:0004625 Certain
      WBbt:0004627 Certain
      WBbt:0004629 Certain
      WBbt:0004647 Certain
      WBbt:0004649 Certain
      WBbt:0004651 Certain
      WBbt:0004653 Certain
      WBbt:0004824 Certain
      WBbt:0004825 Certain
      WBbt:0004826 Certain
      WBbt:0004837 Certain
      WBbt:0004838 Certain
      WBbt:0004840 Certain
      WBbt:0004841 Certain
      WBbt:0004842 Certain
      WBbt:0004858 Certain
      WBbt:0004859 Certain
      WBbt:0004860 Certain
      WBbt:0004861 Certain
      WBbt:0004862 Certain
      WBbt:0004863 Certain
      WBbt:0004865 Certain
      WBbt:0004993 Certain
  Type: Reporter_gene
  Pattern: For lin-39 and mab-5, previous work reported their partially overlapping expression in the ventral cord motor neurons (MNs). In this study, we used two fosmid reporters wgIs18[lin39::EGFP] and wgIs27[mab-5::EGFP] to map their expression to the single-cell resolution among the 75 motor neurons (MNs), which include 54 cholinergic MNs, 19 GABAergic MNs, and 2 serotonergic MNs with stereotypical positions. These MNs can be further classified into eight neuron classes (DA, DB, VA, VB, AS, VC, DD, and VD). By crossing the GFP reporters with mCherry strains labelling cholinergic or GABAergic neurons in the ventral cord, we identified lin-39 expression in DA2-5, DB2-7, VA3-8, VB4-9, AS2-8, VC1-6, DD2-6, and VD3-12 and mab-5 expression in DA4-8, DB5-7, VA6-11, VB8-11, AS5-11, VC3-6, DD2-6, and VD2-12. Both lin-39 and mab-5 expression were generally weaker in more anterior MNs. Interestingly, for cholinergic MNs, mab-5 and lin-39 expression only overlapped in a set of mid-body MNs; MNs more anterior to this region expressed only lin-39 and MNs more posterior expressed only mab-5. For GABAergic MNs (DD and VD), however, lin-39and mab-5 expression overlapped entirely. Outside of the ventral nerve cord, lin-39 was expressed in AQR and AIYL/R in the head and AVM, SDQL/R, PDEL/R, and PVDL/R neurons along the body; mab-5 was expressed in the AVL in the head, SDQL in the mid-body region, and PQR neuron in the tail.
  Reference: WBPaper00062521
  Transgene: WBTransgene00015850