WormBase Tree Display for Expr_pattern: Expr16422
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| Expr16422 | Expression_of | Gene | WBGene00011508 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00011508 | |||
| Expression_data | Anatomy_term | WBbt:0008354 | Certain | |
| Type | Reporter_gene | |||
| Pattern | An mtre-1::GFP transcriptional reporter is expressed downstream of MAB-3/DMD-3 activity in the male tail tip hypodermal cells for a brief period of time at the very end of retraction.Interestingly, transcriptional GFP reporter transgenes for mtre-1 are preferentially expressed in L4 male tail tips at the end of tail tip retraction. Specifically, 75% (27/36) of wild-type L4 males displayed high levels of mtre-1::GFP expression at the end of tail tip retraction, 19% (7/36) displayed low levels of expression and 6% (2/36) failed to show expression. In contrast, 81% (30/37) of wild-type L4 hermaphrodites failed to show any mtre-1::GFP expression in the tail tip, with the remaining 19% (7/37) only showing faint expression. To more closely examine the dynamics of mtre-1::GFP expression, we followed single wild-type L4 males throughout the process of retraction. We observe that mtre1::GFP expression in the male tail tip is restricted to a short 3- to 4-hour window that initiates after tail tip retraction is almost completed and terminates at the onset of anterior tail retraction. In contrast, we did not observe high levels of expression in wild-type L4 hermaphrodites at any time point. In addition, we did not observe any other major regions of expression of mtre1::GFP in either L4 males or hermaphrodites. | |||
| Reference | WBPaper00066057 | |||
| Transgene | WBTransgene00033679 |
