WormBase Tree Display for Expr_pattern: Expr16424
expand all nodes | collapse all nodes | view schema
| Expr16424 | Expression_of | Gene | WBGene00003622 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003622 | ||||
| Expression_data | Life_stage | WBls:0000038 | |||
| WBls:0000041 | Anatomy_term | WBbt:0005319 | |||
| WBls:0000686 | Anatomy_term | WBbt:0005753 | |||
| WBbt:0007809 | |||||
| WBbt:0007846 | |||||
| WBls:0000063 | Anatomy_term | WBbt:0006799 | |||
| Anatomy_term | WBbt:0005319 | Certain | |||
| Life_stage | WBls:0000041 | ||||
| WBbt:0005753 | Certain | ||||
| Life_stage | WBls:0000686 | ||||
| WBbt:0006799 | Certain | ||||
| Life_stage | WBls:0000063 | ||||
| WBbt:0007809 | Certain | ||||
| Life_stage | WBls:0000686 | ||||
| WBbt:0007846 | Certain | ||||
| Life_stage | WBls:0000686 | ||||
| GO_term | GO:0005634 | ||||
| GO:0097598 | |||||
| Type | Genome_editing | ||||
| Reporter_gene | |||||
| Pattern | We found a similar pulse of NHR-23::mScarlet in L4.3 vulval precursor cells as that reported by Kinney et al.. nhr-23::mScarlet was also detectable in seam and hypodermal cells at this stage, epithelial cells that synthesize cuticular components. NHR-23::mScarlet was detected in pachytene nuclei in L4 animals and its zone of expression became restricted in young adult animals. This expression pattern is again similar to NHR-23::GFP::AID*::3xFLAG (Ragle et al. 2020). However, we also observed some interesting differences compared to NHR-23::GFP::AID*::3xFLAG. In our previous work, we observed that NHR::23::GFP became undetectable in ovulating adults (Ragle et al. 2020). In contrast, NHR-23::mScarlet appears to be diffusely expressed in young adult residual bodies and spermatocytes. This diffuse expression persists into ovulating adults, and appears restricted to the spermatheca. The NHR-23::mScarlet signal is specific to nhr-23::mScarlet::3xMyc animals as no diffuse expression in the germline is observed in wild-type animals. One can observe the diffuse expression pattern in L4.5 germlines several rows of cells after NHR-23::mScarlet::3xMyc localization is lost from nuclei. | ||||
| Picture | WBPicture0000015244 | ||||
| Reference | WBPaper00066055 | ||||
| Variation | WBVar02158868 |
