Consistent with previous reports, nfya-1 was expressed in many neurons in the head of worms. We found that nfya-1 was co-expressed with flp-3 in all IL1s at either the adult or larval developmental stage animals, indicating that nfya-1 is indeed expressed in IL1s throughout development. Moreover, GFP-tagged NFYA-1 driven under the control of the nfya-1 promoter was localized to the nucleus of all IL1 neurons, supporting its predicted role as a transcription factor.