WormBase Tree Display for Expr_pattern: Expr1679
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| Expr1679 | Expression_of | Gene | WBGene00002978 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002978 | ||
| Expression_data | Life_stage | WBls:0000022 | |
| WBls:0000016 | |||
| WBls:0000017 | |||
| WBls:0000021 | |||
| WBls:0000010 | |||
| WBls:0000013 | |||
| Anatomy_term (12) | |||
| Type | Reporter_gene | ||
| Pattern | The constructs pTMZIV4349 and pTMZIV1957 induced beta-galactosidase expressions in both the pharyngeal muscles and intestinal cells with similar intensities. Specifically, pharyngeal expressions were observed in all the eight muscle cells (m1-m8), one marginal cell (mc), one epithelial cell (e1) and the four cells of the pharyngo-intestinal valve (PIV). The 20 intestinal cells, some of which are binucleated and localized alongside the intestine, posterior of pharynx and anterior of anus were all stained with intense staining occurring at the most posterior end. Intestinal staining was limited only to intestinal cells, although there were slight variations in position of nuclei and staining intensity. Expression was also detected in embryos between gastrulation and the comma stage. At this stage of development, the exact nuclei are difficult to identify, but the positions and topology suggest pharynx and intestines. By the pretzel stage, identification of the different pharyngeal muscles showing expression becomes possible. A similar expression was also observed in the pharynx of males, although the intestinal staining was more restricted to the posterior region. No expression was induced by the further deletion construct pTMZIV1219. In all cases, the most uniform and intense expression patterns were observed between L1 and L2 worms, and were completed by L2. From L3 to adult, there was a reduction in expression intensity. Both pharyngeal and intestinal expressions were evident within six hours after staining. | ||
| Picture | WBPicture0000010187 | ||
| Remark | This Expr_pattern is about CeTMIV, an isoform of tmy-1 transcription. | ||
| To confirm the CeTMIV isoform expression pattern, corresponding tmy-1::gfp vectors were assayed. Similar GFP expressions induced in pharynx and intestines respectively. These results show that the CeTMIV isoform was expressed in the pharyngeal muscles and intestinal cells and establish that the primary promoter region was located within 853 bp upstream of the initial ATG. | |||
| pretzel stage (author) = fully-elongated embryo (wjc). | |||
| Reference | WBPaper00004944 | ||
| Transgene | WBTransgene00027606 |
