WormBase Tree Display for Expr_pattern: Expr1736
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| Expr1736 | Expression_of | Gene | WBGene00003221 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003221 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000057 | |||
| Anatomy_term (58) | |||
| GO_term | GO:0005634 | ||
| GO:0005694 | |||
| GO:0005654 | |||
| Subcellular_localization | MES-3 is localized predominantly in nuclei. The immunolocalization pattern of MES-3 was analyzed in embryos, using confocal microscopy. Cells at different stages of mitosis were stained by affinity-purified anti-MES-3 antibody and anti-penta-acetylated H4 antibody to visualize chromosomes. During interphase and prometaphase, when condensed chromosomes are clearly visible in nuclei, MES-3 protein is not obviously concentrated on chromosomes; instead it appears evenly distributed in the nucleoplasm. During metaphase and early anaphase, when nuclear envelopes are broken down, some MES-3 protein is detectably associated with chromosomes. | ||
| Type | Antibody | Rabbit polyclonal peptide antibodies against the C-terminal peptide of MES-3 were produced and the distribution of MES-3 was determined by immunostaining. | |
| Pattern | In early embryos, MES-3 protein is present in the nuclei of all cells. As embryogenesis progresses, staining gradually diminishes in somatic cells. In late embryos and L1 larvae, MES-3 is detectable in some somatic cells but is most prominent in Z2 and Z3, the primordial germ cells. The nuclear staining of MES-3 is reduced below detection in any of the four nonconditional alleles of mes-3. | ||
| In wild type adults, MES-3 is most prominent in germline nuclei and is occasionally barely detectable in intestinal nuclei. In the germline, it is present at low levels in distal mitotic nuclei, undetectable in the pachytene region of the distal arm, and present at elevated levels in the proximal meiotic region and in oocytes. | |||
| Picture | WBPicture0000011761 | ||
| WBPicture0000011762 | |||
| Remark | early embryo (author) = blastula embryo (curator) --wjc. | ||
| Reference | WBPaper00005019 | ||
| Antibody_info | WBAntibody00000437 |
