WormBase Tree Display for Expr_pattern: Expr1746
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| Expr1746 | Expression_of | Gene | WBGene00006604 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006604 | |||
| Expression_data | Life_stage | WBls:0000038 | ||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005175 | Certain | ||
| WBbt:0005772 | Certain | |||
| WBbt:0005784 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| Subcellular_localization | Myc-TRA-1A is nuclear and cytoplasmic, with hermaphrodites having higher nuclear TRA-1 than males. | |||
| TRA-1 is both nuclear and cytoplasmic in hermaphrodite and male intestines and germlines. However, in the germline, the levels of nuclear TRA-1 differ depending on the location of the germ cell within the gonad. | ||||
| Type | Antibody | A rabbit peptide polyclonal antibody to the amino terminus of TRA-1 (amino acid 45-62) was used to analyze its location within the cells of intestines and germlines. The peptide antibody recognizes both TRA-1A and TRA-1B. | ||
| Reporter_gene | ||||
| Pattern | In the germline of adult males, TRA-1 localization has some similarities and some important differences with that of hermaphrodites. Like hermaphrodites, TRA-1 is both nuclear and cytoplasmic throughout the germline. However, high levels of nuclear TRA-1 are found only in the mitotic zone. Costaining of male germlines with GLP-1 antibodies and To-Pro-3 indicated that the increased nuclear TRA-1 is coincident with GLP-1 expression and mitotic cells. NIH image analysis indicated that the high levels of staining in the mitotic zone are similar to those in hermaphrodites. However, in the transition zone and meiotic pachytene region, it is about 38% and 46% lower, respectively, than in hermaphrodites (n=6 germlines per sexual phenotype and about 15 nuclei per germline). | |||
| In the hermaphrodite germline, TRA-1 is both nuclear and cytoplasmic. However, there are important regional differences, with the most distal mitotic region and the transition zone having the highest levels of nuclear TRA-1 and the meiotic pachytene region having the lowest. Costaining the germlines with GLP-1 antibodies and the DNA dye To-Pro-3 confirms the spatial distribution of the TRA-1 patterns. Quantitation of about ten germlines indicates high levels of nuclear TRA-1 are detected in the first 3040 nuclei along the germline axis. TRA-1 staining in the meiotic pachytene region is diffuse and distinct nuclei are not apparent. Using NIH image analysis, the levels of staining between the different zones were measured. Both the mitotic and transition zones had similar levels of TRA-1 staining while the meiotic pachytene region was reduced by approximately 40% (n=5 germlines and 15 nuclei were analyzed per zone). In the most proximal region of the meiotic zone, there is a drop in the overall levels of TRA-1. | ||||
| TRA-1 staining in L4 intestine, like the adult male, is diffuse. tra-2(lf) and tra-2(lf) fem-1(lf) tissues were examined to see whether the distribution of TRA-1 correlates with phenotypic sex. In strong tra-2(lf) XX mutants, TRA-1 nuclear levels are similar to wild-type XO males. In contrast, in tra-2(lf); fem-1(lf) XX double mutants, TRA-1 nuclear levels are similar to wild-type XX hermaphrodites. TRA-1 also has a sex-specific spatial distribution in the germline. | ||||
| The distribution of TRA-1 in the germlines of wild-type XX L4 hermaphrodites, tra-2(lf) and tra-2(lf); fem-1(lf) animals was also analyzed. Similar to the intestine, the distribution of TRA-1 is dependent on the sexual state of the tissue. In L4 hermaphrodite and tra-2(lf) germlines, which make only sperm, TRA-1 staining is similar to adult males; TRA-1 nuclear levels are high only in the mitotic region. However, in tra-2(lf); fem-1(lf) germlines, which make only oocytes, TRA-1 nuclear levels are high across the germline. The fact that TRA-1 nuclear levels are high throughout the germline suggests that fem-1 may regulate nuclear TRA-1 levels in the meitotic pachytene region of wild-type adult hermaphrodites. | ||||
| The distribution of TRA-1 in the intestine and gonad was analyzed by immunohistochemistry. In hermaphrodite and male intestines, TRA-1 is both nuclear and cytoplasmic; however, nuclear TRA-1 levels appear higher in hermaphrodites than in males. In males, the staining is diffuse, indicating an even distribution of TRA-1 between the nucleus and cytoplasm. | ||||
| Picture | WBPicture0000009898 | |||
| WBPicture0000009899 | ||||
| Remark | Expression pattern in other life stages are not mentioned. --wjc. | |||
| Reference | WBPaper00005055 | |||
| Transgene | WBTransgene00027664 | |||
| Antibody_info | WBAntibody00000775 |
