WormBase Tree Display for Expr_pattern: Expr1803
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| Expr1803 | Expression_of | Gene | WBGene00000905 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000905 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000021 | ||||
| WBls:0000032 | ||||
| Anatomy_term | WBbt:0004551 | Certain | ||
| WBbt:0004553 | Certain | |||
| WBbt:0004934 | Uncertain | |||
| WBbt:0004935 | Uncertain | |||
| WBbt:0005319 | Certain | |||
| GO_term | GO:0043025 | |||
| Subcellular_localization | The DAF-9::GFP fusion protein was very finely localized within the cell bodies, whereas GFP driven by the same promoter, but without daf-9 coding sequences, diffused throughout the cell body. | |||
| Type | Reporter_gene | |||
| Pattern | The DAF-9::GFP fusion protein was expressed mainly in a pair of neurons anterior to the nerve ring, and the signal was detected in all stages from late embryos to adult. Expression in embryos was observed only in those which developed at 25C. The intensity of the signal was not noticeably different in stages from L2 to adult, or in the dauer larva, but was fainter in embryos and L1 larvae. Weak expression was also observed in the spermathecae of older adults raised at 25C. Two cells strongly expressing GFP were identified as right and left ventral Inner Labial-1 (IL1) neurons, but right and left ventral URA neurons could not be excluded. In some transgenic animals, DAF-9::GFP was only expressed in one of the ventral cells. The positions of these two cell bodies are variable, with one occasionally found anterior to the metacorpus. | |||
| Remark | When injected into the germline of daf-9(m540), it fully rescued the Daf-c phenotype (Table 3), indicating that the gfp reporter must be expressed in cells required for daf-9 function. | |||
| Reference | WBPaper00005086 | |||
| Transgene | WBTransgene00027696 |
