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WormBase Tree Display for Expr_pattern: Expr2230

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Name Class

Expr2230Expression_ofGeneWBGene00000553
Reflects_endogenous_expression_ofWBGene00000553
Expression_dataGO_termGO:0005634
Subcellular_localizationGFP-fusion constructs of CeCaM-KI was transfected into COS-7 cells. Expression of the GFP-fusion protein of each CaM-KI was confirmed by Western blotting using anti-GFP antibody and the CaM overlay method. CeCaM-KI (wild type, Pro2-Ala348) is highly concentrated in the nucleus. When a mutant CeCaM-KI lacking 6 residues (Pro2-Arg7) at the NH2-terminal region was used, it was no longer staying in the nucleus, suggesting that the NH2-terminal 6 residues contain a potential NLS. GFP CeCaM-KK was detected localized in both the cytoplasm and the nuclei of transfected cells.
RemarkType: mammalian cell transfection.
ReferenceWBPaper00003625