WormBase Tree Display for Expr_pattern: Expr2247
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Expr2247 | Expression_of (2) | |
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Expression_data (2) | ||
Subcellular_localization | NHX-8 appeared to be associated with intracellular membranes. The NHX-8::GFP protein was distributed in the seam cells in a vesicular pattern that excluded the nucleus, with some underlying signal contributed by weakly fluorescent hypodermal syncitia. | |
Type | Reporter_gene | |
Pattern | In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. NHX-8::GFP was expressed as expected in the seam cells but was also expressed in the pharyngeal muscles, the pharyngeal-intestinal and intestinal-rectal valve cells, and in cells of the vulva. Expression in these additional cells may have been a result of promoter or enhancer elements in the first intron of the nhx-8 gene that are not present in the transcriptional fusion construct. Alternatively, the shorter promoter used in the translational reporter construct (1.5 versus 4 kb) may lack sequences that normally repress expression in these cells. See Expr2237 for expression pattern observed with transcriptional fusion construct. | |
Picture | WBPicture0000008208 | |
Reference | WBPaper00005421 | |
Transgene | WBTransgene00027959 |