WormBase Tree Display for Expr_pattern: Expr2554
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| Expr2554 | Expression_of | Gene | WBGene00000804 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000804 | |||
| Expression_data | Anatomy_term | WBbt:0006797 | Certain | |
| GO_term | GO:0005694 | |||
| GO:0051233 | ||||
| Subcellular_localization | In oocytes, during meiosis I, CSC-1 localizes to a discrete region of meiotic bivalents. During anaphase of meiosis I, CSC-1 localizes to the midzone of the meiotic spindle. In mitosis, CSC-1 localizes to chromosomes during metaphase and to the spindle midzone during anaphase and telophase. | |||
| Type | Antibody | Polyclonal peptide rabbit antibody. CSC-1specific antisera were produced in rabbits using a COOH-terminal peptide (CGPLQPETENAGTSV) coupled to keyhole limpet hemocyanin. An antibody raised against an NH2-terminal peptide provided essentially identical results in immunofluorescence and immunoprecipitation assays. Antibodies were affinity purified using immobilized peptides. | ||
| Picture | WBPicture0000008958 | |||
| Remark | The localization of CSC-1 on the central spindle is significantly broader than the localization of ZEN-4, the kinesin-like protein that is part of the centralspindlin complex. The reactivity of antiCSC-1 antibodies on chromatin and the central spindle is abolished in csc-1(RNAi) embryos. All features of the localization of CSC-1 are similar to those described for other members of the ABI complex. | |||
| Reference | WBPaper00005872 | |||
| Antibody_info | WBAntibody00000622 | |||
| WBAntibody00000623 |
