WormBase Tree Display for Expr_pattern: Expr2574
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Expr2574 | Expression_of | Gene | WBGene00003000 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003000 | ||
Expression_data | Life_stage | WBls:0000038 | |
WBls:0000041 | |||
Anatomy_term (18) | |||
Type | Reporter_gene | ||
Pattern | The developmental profile of the lin-11::GFP vulval expression in all three lines is nearly identical, although their fluorescence brightness can be ranked nIs96>syIs80>syIs53. The syIs80 and syIs53 animals reveal dynamic changes in the vulval GFP expression. The earliest GFP expression in syIs80 vulval cells is detected in one of the two daughters of the 2 lineage precursors (P5.pp and P7.pa cells). In most cases, GFP fluorescence was detectable only ~1-2 hours before the VPC daughters were beginning to divide. At this stage, expression in P6.p daughters is much weaker and rarely observed. During the Pn.pxx stage, vulval cells begin to reveal brighter GFP fluorescence in both the 1 and 2 lineages. In the 2 lineage, expression is typically seen in only the N and T cells. By the Pn.pxxx stage, lin-11::GFP expression is detected in all 2 lineage progeny. In general, vulA has the lowest level of expression compared with others. | ||
syIs53 animals reveal a similar pattern of expression, although the overall fluorescence is considerably reduced. By mid-L4 stage, the GFP fluorescence in syIs53 and syIs80 strains begins to fade, and can not be seen by late-L4 stage. However, in nIs96 animals fluorescence can be detected in young adult animals. Expression is also detected in the uterine lineage cells, VC neurons and a subset of the head and tail neurons. In addition, expression in the B.pap and its descendents was observed in the developing male proctodeum. | |||
Picture | WBPicture0000007544 | ||
Remark | No detailed description on expression pattern in other life stage or anatomy parts. | ||
Reporter gene fusion type not specified. | |||
Reference | WBPaper00005954 | ||
Transgene | WBTransgene00004695 | ||
WBTransgene00004675 | |||
WBTransgene00001284 |