WormBase Tree Display for Expr_pattern: Expr2605
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Expr2605 | Expression_of | Gene | WBGene00005008 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00005008 | ||
Expression_data | GO_term | GO:0005634 | |
Subcellular_localization | When expressed in Sf9 insect cells using a baculovirus expression system, SPR-3 is localized in the nucleus. Attempts to purify SPR-3 from insect cells failed. SPR-3 remained in the nuclear fraction even after DNase treatment and high salt extraction. It is therefore likely that SPR-3 is present in a nuclear subcompartment or attached to the nuclear periphery. | ||
Picture | WBPicture0000007543 | ||
Remark | Type: mammalian cell transfection. The Baculovirus expression construct was made by inserting the spr-3 cDNA into the transfer vector pBY1296 fusing a GST-Myc-tag N-terminally to spr-3. | ||
Reference | WBPaper00005903 |