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WormBase Tree Display for Expr_pattern: Expr2872

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Name Class

Expr2872Expression_ofGeneWBGene00006515
Reflects_endogenous_expression_ofWBGene00006515
Expression_dataGO_termGO:0005634
GO:0030496
GO:0005737
GO:0005813
Subcellular_localizationIndirect immunofluorescence with anti-myc antibody showed that myc-APACD was localized both in the cytoplasm and in the nucleus. GFP-APACD was also found in the nucleus and the cytoplasm. Furthermore, GFP-APACD often showed co-localization with a centrosomal marker gamma-tubulin.APACD protein also concentrated on the midbody during cytokinesis.
RemarkType: mammalian cell transfection. mouse APACD cDNA was cloned and expressed myc-tagged or GFP-tagged APACD in HeLa cells.Cell extracts were immunoblotted with anti-myc or anti-GFP antibody.
ReferenceWBPaper00006406