WormBase Tree Display for Expr_pattern: Expr2875
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Expr2875 | Expression_of | Gene | WBGene00001983 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00001983 | |||
Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
Pattern | Approximately two times more hoe-1 mRNA was observed in wild-type worms relative to glp-1(e2141ts) mutant worms. Quantitative real-time PCR analysis of the quantity of hoe-1 mRNA in adult glp-1(oz112) animals revealed a threefold to fourfold increase as compared to wild-type adults. Thus, the level of hoe-1 mRNA roughly correlates with the extent of proliferation of the germ line. | |||
Remark | Type: quantitative, real-time PCR analysis using RNA derived from adult wild-type worms, adult glp-1(e2141ts) mutant worms, which do not produce a germ line when grown at restrictive temperature, and adult glp-1(oz112) animals, which have a tumorous germline phenotype where germ cells never leave the mitotic cell cycle. If hoe-1 is expressed in the germ line, less hoe-1 message was expected to be seen in the glp-1(e2141ts) sample of RNA. | |||
Reference | WBPaper00006401 |