WormBase Tree Display for Expr_pattern: Expr2985
expand all nodes | collapse all nodes | view schema
| Expr2985 | Expression_of | Gene | WBGene00004010 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004010 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000021 | |||
| WBls:0000010 | |||
| WBls:0000013 | |||
| GO_term | GO:0005737 | ||
| Subcellular_localization | Most if not all PHA-1::GFP fusion protein localized to the cytoplasm. This was particularly apparent in larvae, where the contribution of nonspecific GFP fluorescence coming from layers outside of the central focal plane was minimized. No evidence was observed for any dynamic regulation of PHA-1 with respect to its subcellular localization. Although these findings do not rule out the possibility that some PHA-1 protein may normally be present in nuclei, they strongly suggest that PHA-1 resides primarily, if not exclusively, in the cytoplasmic compartment. Moreover, although it was possible that the tagged fusion protein may have been mislocalized, either due to the presence of the C-terminal GFP extension, or simply through overexpression, the ability of the fusion protein to substitute functionally for wild-type PHA-1 suggests that it is localizing to the correct cellular compartment. | ||
| Type | Reporter_gene | ||
| Pattern | Expression of the pha-1 reporter was first detected at the approximately 100-cell stage in all somatic cells of the embryo. This pattern of widespread expression continued through later stages of embryonic development, diminishing somewhat in intensity by the time of hatching. Expression was also observed in early-larval-stage animals and was present but reduced in late-stage larvae and adults. | ||
| Remark | Similar expression patterns were also observed using reporter constructs containing approximately 2.9 kb of upstream regulatory sequences together with genomic sequences encoding the first 137 aa of PHA-1 fused to a GFP containing a nuclear localization signal. | ||
| Reference | WBPaper00024215 | ||
| Transgene | WBTransgene00028290 |
