WormBase Tree Display for Expr_pattern: Expr3078
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| Expr3078 | Expression_of | Gene | WBGene00003473 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003473 | |||
| Expression_data | Anatomy_term | WBbt:0003732 | Certain | |
| WBbt:0005772 | Certain | |||
| Type | Reporter_gene | |||
| Pattern | The major location of the cadmium inducible metallothionein expression was strictly confined to the intestine, mtl-1 was constituently expressed, even in the absence of cadmium, in the second pharyngeal bulb. | |||
| Remark | The GFP signal could be turned on by exposing nematodes to metals. The overall response characteristics mirrored the data obtained from the semi-quantitative PCR, namely the minimum response concentration was 2.5 M cadmium over a 24 hour exposure period and, as before, copper and zinc were poor inducers. Short-term exposures of one hour required a cadmium concentration in excess of 20 M to initiate a strong positive GFP signal. Although there was no observable isoform specific divergence in signal intensity, dose or time response, the cellular localisation differed to some extent. | |||
| Reference | WBPaper00024351 | |||
| Transgene | WBTransgene00028340 |
