WormBase Tree Display for Expr_pattern: Expr3111
expand all nodes | collapse all nodes | view schema
| Expr3111 | Expression_of | Gene | WBGene00004952 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004952 | ||
| Expression_data | GO_term | GO:0005634 | |
| GO:0051233 | |||
| GO:0005874 | |||
| Subcellular_localization | SPD-1::GFP initially localized to pro-nuclei as they migrated and met in the one cell embryo. Prior to visible nuclear breakdown, the signal in the nucleus began to diminish, and there was a slight increase in cytoplasmic signal and a faint accumulation of signal around the centrosomes. As the spindle assembled, there was a diffuse staining on the spindle with a marked absence on chromosomes at the metaphase plate. SPD-1 underwent a rapid mobilization after the metaphase-anaphase transition. A bright signal appeared at the spindle midzone in a restricted region, similar to the region where other midzone components have been seen to localize. The signal around the centrosomes increased, and linear structures appeared in the cytoplasm. These structures were not as bright as the structures associated with the midzone microtubules. To determine whether these structures corresponded to microtubules, embryos were fixed and stained with GFP and tubulin antibodies and found that SPD-1::GFP colocalized to microtubules along a part of their length. These are probably bundles of anti-parallel microtubules. SPD-1::GFP began to accumulate in the nuclei as soon as these structures visibly reformed. As the furrow ingressed, the spindle midzone signal was compacted from individual bundles into one larger structure. This same pattern was observed in subsequent divisions, including EMS divisions. Rabbit polyclonal antibodies raised and purified against SPD-1 also localized to nuclei and the spindle midzone | ||
| Type | Reporter_gene | ||
| Picture | WBPicture0000009774 | ||
| Reference | WBPaper00024415 | ||
| Transgene | WBTransgene00001395 | ||
| Antibody_info | WBAntibody00000779 |
