WormBase Tree Display for Expr_pattern: Expr4653

Expr4653 Expression_of: Gene: WBGene00001481
    Reflects_endogenous_expression_of: WBGene00001481
  Expression_data: Life_stage: WBls:0000038
      WBls:0000027
      WBls:0000035
    Anatomy_term: WBbt:0005784 Certain
  Type: Antibody
  Pattern: In L4 germ lines, authors also examined SP56, the earliest known marker of sperm differentiation: SP56 antibodies recognize a minor sperm protein in primary, secondary, and mature spermatocytes. SP56 was first seen approximately 6 hr after the molt from L3 to L4 (3 hr, n = 17; 6 hr, n = 14; 10 hr, n = 19), where staining was limited to the proximal-most germ cells. As animals matured through the L4 stage, the SP56 domain expanded distally to include an increasing number of cells. Importantly, FOG-1 and SP56 were not present in the same cells at the same time. Instead, FOG-1 staining preceded SP56 staining in both time and space. For example, the proximal-most germ cells expressed FOG-1 in late L3 and early L4 ; those same cells expressed SP56 in mid-L4 (6 hr), when the FOG-1 domain had moved distally. Authors conclude that in wild-type larvae, FOG-1 is expressed transiently in germ cells destined for spermatogenesis.
    In wild-type larvae, FOG-1 became easily detectable in the second half of L2. In early L3 larvae, FOG-1 levels increased in the proximal region of most germ lines, and by late L3, FOG-1 staining was strong in that proximal region of all germ lines. FOG-1 remained abundant for approximately the first half of the L4 stage, although its location moved distally within the germ line.
  Remark: No detailed description expression patterns in other life stages.
  Reference: WBPaper00029090
  Antibody_info: WBAntibody00000874