WormBase Tree Display for Expr_pattern: Expr4759
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| Expr4759 | Expression_of | Gene | WBGene00018798 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00018798 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| Anatomy_term | WBbt:0005451 | Certain | ||
| WBbt:0005772 | Certain | |||
| WBbt:0006797 | Certain | |||
| WBbt:0006798 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | Ce-GnRHR was localized to the nucleus of maturing oocytes and intestinal cells, to sperm, pharyngeal muscle. | |||
| Type | Antibody | |||
| Pattern | To better localize cellular staining in worms, authors permeabilized worms and performed whole-mount fluorescent immunohistochemistry. These experiments indicated that Ce-GnRHR was localized to the nucleus of maturing oocytes and intestinal cells, to sperm, pharyngeal muscles, but not other cells such as hypodermal cells. Similar staining of these structures was evident for both the anti-human-GnRHR1 and anti-Ce-GnRHR antibodies. Ce-GnRHR staining clearly illustrates an increase in the size of the oocyte nuclei as they mature along the gonadal arm. Upon fertilization, Ce-GnRHR staining becomes diffuse throughout the developing egg, although staining appears to increase during egg development. Only uniform background autofluorescence was apparent in worms treated with secondary antibody alone. Ce-GnRHR also was detected along the myofilament lattice of the pharyngeal muscles. staining along the three parallel muscles that comprise the pharyngeal musculature beginning at the tip of the head and extending to the pharyngeal bulb, staining of all 8 pharyngeal muscle (pm1 to 8) domains, but not in the gaps between contractile zones of adjacent pharyngeal muscle domains. staining of myofilaments that run radially towards the lumen and which are most obvious in the bulbs. These results indicate Ce-GnRHR is present on the pharyngeal musculature. | |||
| Picture | WBPicture0000012082 | |||
| WBPicture0000012083 | ||||
| Remark | The specificity of binding of anti-Ce-GnRHR antibody to GnRHR was demonstrated by the lack of staining throughout the worm (including the germline, pharyngeal muscle and intestinal cells) when the antibody was preincubated with its antigen (the C-terminus amino acids 386 to 401). | |||
| Reference | WBPaper00028848 | |||
| Antibody_info | WBAntibody00001186 |
