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WormBase Tree Display for Expr_pattern: Expr518

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Name Class

Expr518Expression_ofGeneWBGene00001263
Reflects_endogenous_expression_ofWBGene00001263
Expression_dataLife_stageWBls:0000024
WBls:0000038
WBls:0000027
WBls:0000035
WBls:0000041
WBls:0000021
WBls:0000013
Anatomy_term (17)
GO_termGO:0005604
Subcellular_localizationPreceding lima stage, intracellular. Later, extracellular around muscle quadrant-hypodermis interface, pharynx, intestine and gonad. Gonad and muscle stain intracellularly, pharynx and hypodermis do not. Four antibodies to type IV collagen gave same expression pattern.
TypeAntibodyanti-EMB-9 (type IV collagen)
Reporter_gene
PatternBody wall muscle cells, hmc and MS.pppaaa show expression in early embryo. In later embryo through adulthood, stain accumulates between the hypodermis and four body muscle quadrants, and surrounds the pharynx, intestine and primordial gonad at L1 through adulthood. Presumptive GLRDL, GLRDR, GLRL, GLRR, GLRVL, GLRVR, coelomocytes, and anal muscles show expression in addition to body muscle cells at L1, L2, L3 L4 and adult. Distal tip cells of developing gonad in L2, L3, and L4. Spermathecal cells and distal ends of gonad show expression at L4 and adult. Vulval and uterine muscles in adult. Also expressed in two to four posterior intestinal cells.
PictureWBPicture0000008895
WBPicture0000008898
WBPicture0000008899
RemarkReporter construct derived from clones that rescue emb-9 phenotype. Transgenic Marker: rol-6(su1006).
late embryo(author) = fully-elongated embryo(curator).
ExperimentStrainWBStrain00000001
ReferenceWBPaper00002813
TransgeneWBTransgene00029622
Antibody_infoWBAntibody00000146
WBAntibody00001813