WormBase Tree Display for Expr_pattern: Expr575
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| Expr575 | Expression_of | Gene | WBGene00004027 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004027 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000005 | ||||
| Anatomy_term | WBbt:0004421 | |||
| WBbt:0004422 | ||||
| WBbt:0004575 | ||||
| WBbt:0004576 | ||||
| WBbt:0005784 | Certain | |||
| WBbt:0006797 | ||||
| WBbt:0006873 | ||||
| WBbt:0006874 | ||||
| WBbt:0006875 | ||||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| GO:0005813 | ||||
| GO:0043186 | ||||
| Subcellular_localization | A subset of PIE-1 molecules are associated with P granules, but there is a diffuse cytoplasmic component of PIE-1 staining in contrast to the punctate P granule pattern. PIE-1 protein is cytoplasmic until the 2-cell stage, after which it is increasingly nuclear. PIE-1 protein distribution in P0, P1, P2 and P3 follows a similar pattern: initially uniform cytoplasmic distribution, followed by asymmetric cytoplasmic localization at the time of P granule migration, and asymmetric cytoplasmic and centralsomal localizations during mitosis. | |||
| Type | Antibody | anti-PIE-1 mouse monoclonal antibodies | ||
| In_situ | Digoxigenin labeled DNA probes | |||
| Pattern | In oocytes,1-cell and 2-cell stage embryos, pie-1 mRNA is distributed uniformly. After the 4-cell stage, pie-1 mRNA was lost from somatic blastomeres and remained in the germ lineage. PIE-1 protein was first detected in maturing oocytes, where it is uniformly distributed. PIE-1 level increase during the one cell stage. PIE-1 protein also exists in P1, P2 P3, P4, Z2 and Z3. In P4, PIE-1 is found equally in the P4 daughters (Z2 and Z3) in contrast to earlier divisions in which PIE-1 is localized to the posterior cytoplasm and posterior centrosomes during mitosis. | |||
| Remark | early embryo(author) = blastula embryo(curator). | |||
| Experiment | Strain | WBStrain00000001 | ||
| Reference | WBPaper00003175 | |||
| Antibody_info | WBAntibody00000189 |
