WormBase Tree Display for Expr_pattern: Expr7858

Expr7858 Expression_of: Gene: WBGene00004101
    Reflects_endogenous_expression_of: WBGene00004101
  Expression_data: Life_stage: WBls:0000024
      WBls:0000003
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term: WBbt:0003681 Certain
      WBbt:0005178 Certain
      WBbt:0005319 Certain
      WBbt:0005733 Certain
      WBbt:0005751 Certain
      WBbt:0005772 Certain
      WBbt:0005775 Certain
      WBbt:0006748 Certain
      WBbt:0006760 Certain
    GO_term: GO:0010008
  Subcellular_localization: The protein was detected as a punctate pattern in the cytoplasm indicating a vesicular localization. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells, where they localized close to the apical membrane as revealed by the apical junction marker AJM-1. Later during morphogenesis, CeVPS-27-positive vesicles became more uniformly localized. Several arguments support the idea that CeVPS-27 localizes to endosomes (see Remark).
  Type: Antibody
    Reporter_gene
  Pattern: CeVPS-27 was detected ubiquitously, as early as in the 2-cell embryo and persisted throughout the embryonic and larval development and in adult animals. At mid-embryogenesis, when epithelialization proceeds, CeVPS-27-positive vesicles were preferentially enriched in hypodermal, pharyngeal and intestinal cells. The specificity of the signal was confirmed by its complete disappearance after inactivation of Cevps-27 by RNAi. During larval development and in adults, CeVPS-27 was expressed ubiquitously but remained more abundant in the intestine, the hypodermis, the pharynx and in the adult epithelial tissues; namely the uterus, the vulva, the spermatheca and the gonad. CeVPS-27 could also be found in the excretory canal, and the six scavenger cells called coelomocytes.
  Remark: Cevps-27 = C07G1.5
    Immunohistochemical analyses with anti-CeVPS-27 antibodies (Figure 2AD,GJ) and observation of CeVPS-27::GFP transgenic worms (Figure 2E,F,K) revealed a similar expression pattern.
    Picture: Figure 2, Figure 3.
    Several arguments support the idea that CeVPS-27 localizes to endosomes. First, CeVPS-27-positive vesicles are not lysosomes as revealed by an absence of colocalization of CeVPS-27::GFP with the lysosomal marker lysotracker. Second, CeVPS-27 is localized in vesicles close to the plasma membrane in epithelial cells and in the coelomocytes, which are specialized in endocytosis of fluid and macromolecules from the body cavity. Finally, the inactivation of the homolog of the class E vps-23, which in yeast and mammals results in the formation of enlarged endosomes, induced the production of enlarged CeVPS-27-positive vesicles. Together, these data indicate that CeVPS-27 is localized at the endosomal membrane in C. elegans.
  Reference: WBPaper00026641
  Transgene: WBTransgene00030161
  Antibody_info: WBAntibody00001021