WormBase Tree Display for Expr_pattern: Expr8329
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| Expr8329 | Expression_of | Gene | WBGene00003169 |
|---|---|---|---|
| WBGene00003166 | |||
| WBGene00003168 | |||
| Reflects_endogenous_expression_of | WBGene00003166 | ||
| WBGene00003168 | |||
| WBGene00003169 | |||
| Expression_data | GO_term | GO:0032589 | |
| Subcellular_localization | Anti-MEC-4 and anti-MEC-2 antibodies decorated two structures in wild-type TRNs, the plasma membrane and the 15pf microtubules, whereas anti-MEC-5 antibodies decorated the space in between TRN neurites and the surrounding hypodermal cell. Approximately half of the MeT channel labels were associated with the plasma membrane: 51% (n = 49) and 44% (n = 36) of anti-MEC-2 and anti-MEC-4 labels, respectively). These labels were distributed around the circumference of the TRN neurite without any obvious bias for either the apical or basal aspect of the neurite. In some cases, clusters of gold labels were associated with the plasma membrane. Half of the membrane-associated anti-MEC-4 labels were doublets, whereas approximately one-fifth of the membrane-associated anti-MEC-2 labels contained clusters of 2 or 3 gold beads. Anti-MEC-5 antibodies were significantly more likely to be found on the apical side of TRN neurites. The remaining MeT channel labels were associated with 15pf microtubules distributed throughout the cytoplasm. Intracellular labels were not associated with vesicles in TRN neurites and vesicles were not detected in any of the five SS-IEM reconstructions, which covered a total length of 63 um. | ||
| Type | Antibody | Electron micrograph | |
| Picture | WBPicture0000011150 | ||
| Remark | Picture: Fig 3, S1, Supplementary Video 1 | ||
| Reference | WBPaper00031345 | ||
| Antibody_info | WBAntibody00001354 | ||
| WBAntibody00001355 | |||
| WBAntibody00001356 |
