WormBase Tree Display for Expr_pattern: Expr8651

Expr8651 Expression_of: Gene: WBGene00001132
    Reflects_endogenous_expression_of: WBGene00001132
  Expression_data: Life_stage: WBls:0000024
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
    Anatomy_term: WBbt:0003681 Partial
      WBbt:0005813 Certain
    GO_term: GO:0055120
  Subcellular_localization: To determine in which sub-region ALP-1 proteins are located, we colabeled body wall muscle with antibodies directly against ALP-1(B78) and vinculin. Double labeling with anti-ALP-1 and anti-vinculin antibodies revealed both proteins are localized at dense bodies, but ALP-1 is absent from muscle cellcell junctions (dense plaques) that contain vinculin. High magnification views of the dense bodies revealed that ALP-1 proteins colocalize with anti-actinin, but only have limited overlap with vinculin. These data indicate ALP-1 and -actinin colocalize within the dense body in a region that is spatially distinct from the basal, membrane proximal zone that accumulates vinculin.
    Within body wall muscle, anti-ALP-1 staining appeared in a periodic punctate array that was identified as dense bodies by colabeling with an anti-actinin antibody. It should be noted that in body wall muscle, using anti-ALP-1 antibodies authors did not observe any nuclear staining, such as that reported for the ALP-1::GFP translational reporter.
  Type: Antibody
  Pattern: Immunostaining of wild-type worms using B74 or B78 revealed that endogenous ALP-1 proteins are highly expressed in the body wall muscle throughout postembryonic development. In addition, B78 (against ALP-1A, B, and D) also detects ALP-1 proteins at the apical and basal surfaces of the pharynx, another musculature structure in the worm, but at a very low level of expression.
  Picture: WBPicture0000008861
  Remark: Picture: Figure 2.
  Reference: WBPaper00032968
  Antibody_info: WBAntibody00001942
    WBAntibody00001943