WormBase Tree Display for Expr_pattern: Expr8775
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| Expr8775 | Expression_of | Gene | WBGene00014251 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00014251 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0005733 | Certain | ||
| WBbt:0005772 | Certain | |||
| WBbt:0005799 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005777 | |||
| Subcellular_localization | The presence of a peroxisomal targeting signal in its C-terminus and the punctate localization pattern suggest that GSTK-1 is a peroxisomal protein. To confirm the peroxisomal localization, the peroxisomal targeting signal (PTS1) was masked by fusing the GFP at the C-terminus of GSTK-1. Transgenic worms for GSTK-1::GFP only presented diffuse staining, further supporting a peroxisomal localization of GSTK-1. | |||
| To confirm mitochondrial localization, both GFP::GSTK-1 and GSTK-2::GFP-expressing worms were stained with MitoTracker Red. GFP::GSTK-1 did not show any colocalization with MitoTracker Red staining. | ||||
| Type | Reporter_gene | |||
| Pattern | The expression of the gfp::gstk-1 transgene was first detected in 100 cell embryos. Expression was increased during morphogenesis of the embryo. Fluorescence was observed as strong punctate staining in intestinal cells and in the epidermis. The number and intensity of fluorescent structures increased strongly in the intestine during larval development, whereas the epidermal fluorescent punctata weakened. In addition, a diffuse localization of GFP::GSTK-1 was observed in the body wall muscles and in the rectal gland cells in larvae and adults. | |||
| Remark | Picture: Fig 2, Fig. 3. | |||
| Reference | WBPaper00035091 | |||
| Transgene | WBTransgene00030704 |
