WormBase Tree Display for Expr_pattern: Expr8817
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| Expr8817 | Expression_of | Gene | WBGene00019629 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00019629 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0043186 | |||
| GO:0048471 | ||||
| Subcellular_localization | CDE-1 was detected throughout the female germline cytoplasm, where it was enriched in the perinuclear region. However, CDE-1 did not display the clear granular structure that is commonly observed for the P granule marker, PGL-1 at this stage. In the sperm-producing germlines of both males and L4 stage hermaphrodites, bright granules were found in close proximity to the condensing DNA of maturating sperm. In embryos, CDE-1 was detected in perinuclear granules in the P-cell lineage, mainly colocalizing with PGL-1. In addition, staining could be detected on the outer edges of condensing chromosomes at prometaphase. This staining became more intense on the spindle-facing sides of the metaphase plate and remained detectable through anaphase. Parallel experiments in cde-1 mutant animals did not show the patterns described above, indicating that the observed signals reflect true CDE-1 localization. Specific intestinal staining as previously reported based on a transgenic approach (see Expr3928) could not be detected. | |||
| Type | Antibody | |||
| Picture | WBPicture0000009464 | |||
| Remark | Picture: Figure 2. | |||
| Reference | WBPaper00035269 | |||
| Antibody_info | WBAntibody00002013 | |||
| WBAntibody00002014 |
