WormBase Tree Display for Expr_pattern: Expr8837
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| Expr8837 | Expression_of | Gene | WBGene00020035 | |
|---|---|---|---|---|
| WBGene00020652 | ||||
| Reflects_endogenous_expression_of | WBGene00020035 | |||
| WBGene00020652 | ||||
| Expression_data | Anatomy_term | WBbt:0006797 | Certain | |
| GO_term | GO:0005634 | |||
| GO:0005938 | ||||
| Subcellular_localization | EGG-4/5 localize to the cortex of oocytes. In addition, EGG-4/5 were also detected in oocyte nuclei. The cortical and nuclear signals were eliminated by RNAi codepletion of EGG-4 and EGG-5, confirming that they correspond to endogenous EGG-4/5. Staining of hermaphrodites homozygous for single deletions in either EGG-4 or EGG-5 gave the same pattern as wild-type, confirming that EGG-4 and EGG-5 are both expressed in oocytes. In zygotes in meiosis II, EGG-4/5 were enriched on subcortical puncta, also positive for EGG-3. EGG-3-positive puncta invade the cytoplasm during meiosis II. EGG-4/5 puncta, in contrast, were prominent only near the cortex and around the sperm pronucleus. No EGG-4/5 were detected above background in 2-cell embryos. | |||
| Type | Antibody | |||
| Picture | WBPicture0000009477 | |||
| WBPicture0000009478 | ||||
| Remark | Picture: Figure 4B, 4C, Figure S6 | |||
| Reference | WBPaper00035427 | |||
| Antibody_info | WBAntibody00002049 |
