WormBase Tree Display for Expr_pattern: Expr8896
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| Expr8896 | Expression_of | Gene | WBGene00011910 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00011910 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0005737 | |||
| GO:0043186 | ||||
| Subcellular_localization | In GFP::alg-3 hermaphrodites and males, GFP expression was observed in the proximal germline beginning at the L4 stage, coincident with the onset of spermatogenesis. In adult hermaphrodites, after the switch to oogenesis, GFP::ALG-3 expression was restricted to the spermatheca. Within the spermatheca, in hermaphrodites and testis in males, GFP::ALG-3 was localized within residual bodies, which are enucleate cytoplasts produced by the budding off of mature spermatids at the end of meiosis II. Little if any GFP::ALG-3 could be detected in mature spermatids. As expected, the proximal expression of GFP::ALG-3 continued through adulthood in males. Within the developing spermatocytes, GFP::ALG-3 was first apparent in the postpachytene germline before the point where chromosomes appear to aggregate into a single mass. GFP signal was localized throughout the cytoplasm and enriched at perinuclear foci coincident with P granules based on colocalization with RFP::PGL-1. | |||
| Type | Reporter_gene | |||
| Picture | WBPicture0000010889 | |||
| WBPicture0000010890 | ||||
| Remark | Picture: Fig. 2, Fig S3A, S3B. | |||
| Reference | WBPaper00035890 | |||
| Transgene | WBTransgene00030781 |
