WormBase Tree Display for Expr_pattern: Expr9076
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Expr9076 | Expression_of | Gene | WBGene00009098 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00009098 | |||
Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
WBbt:0005319 | Certain | |||
WBbt:0005753 | Certain | |||
WBbt:0005772 | Certain | |||
WBbt:0005813 | Certain | |||
WBbt:0005828 | Certain | |||
WBbt:0006749 | Certain | |||
GO_term | GO:0016323 | |||
GO:0016324 | ||||
GO:0010008 | ||||
GO:0031901 | ||||
GO:0055038 | ||||
Subcellular_localization | In the C. elegans intestine, RFP-RAB-10 and GFP-RAB-8 colocalized extensively. Closer examination showed that EHBP-1 colocalized with RAB-10 and RAB-8 on punctate endosomal membranes. EHBP-1 also localized to tubular elements associated with the puncta and the plasma membrane. EHBP-1-GFP also occasionally overlapped with the early endosome marker RFP-RAB-5, but the EHBP-1-GFP labeled structures more often localized near RFP-RAB-5 puncta rather than overlapping with them directly. EHBP-1-GFP was not found to colocalize with the apical recycling marker RFP-RAB-11 or the Golgi marker MANS-RFP. Taken together these results indicate that EHBP-1 is enriched on endosomes. | |||
The EHBP-1-GFP fusion protein was localized in a punctate or tubular pattern in most tissues. In the intestine, EHBP-1-GFP was localized to both the apical and basolateral plasma membranes. Near basolateral membranes, EHBP-1-GFP labeled distinct cytoplasmic puncta resembling endosomes ranging in size from 0.5 to 1.0 um. In addition to punctate structures, EHBP-1-GFP also labeled tubular structures close to the basolateral plasma membrane of the intestine. | ||||
Type | Reporter_gene | |||
Pattern | EHBP-1-GFP fusion proteins were expressed in all somatic C. elegans tissues, including the pharynx and nerve ring, body-wall muscles, seam cells, intestine, oviduct sheath cell and spermatheca. | |||
Picture (2) | ||||
Remark | Picture: Fig 2, Fig 3, Fig S4. | |||
Reference | WBPaper00036404 | |||
Transgene | WBTransgene00030890 |