WormBase Tree Display for Expr_pattern: Expr9123
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| Expr9123 | Expression_of | Gene | WBGene00003285 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003285 | ||
| Expression_data (2) | |||
| Type | Reporter_gene | ||
| Pattern | Examination of larvae and adults showed that the reporter expression remained confined to the posterior of the animal with the exception of weak expression in more anterior intestinal cells. Signal appears to increase through the L2 stage, after which it decreases with only minimal levels detectable in the tails of adults in hermaphrodites. By contrast in males the adult tail shows high levels of expression. | ||
| Stably integrated lines with a 2.26 kb fragment upstream of the mir-57 mature sequence fused with a fluorescent reporter mCherry showed expression in the posterior cells of the embryo in a variety of tissues. Automated analysis of 3D time-lapse movies followed by manual editing revealed that the reporter was expressed in a bilaterally symmetric pattern in the posterior daughters of sublineages of AB and C founder cells, beginning at about the 200-cell stage. The cells from these sublineages lie in the posterior part of the embryo only and represent a wide variety of cell types, including tail seam cells, the hypodermal cells hyp10 and hyp11, the cells producing the tail spike, rectal cells, the P11/12 cells and even body wall muscle cells. Inspection of the movies beyond the comma stage also showed expression in the intestinal cells after elongation. | |||
| Picture | WBPicture0000007469 | ||
| Remark | A total of 12 independent transgenic lines were generated via bombardment and all of them showed the similar expression patterns. | ||
| Picture: Fig 1, Fig S1, Fig S2. | |||
| Reference | WBPaper00037133 | ||
| Transgene | WBTransgene00007725 |
