WormBase Tree Display for Expr_pattern: Expr9631
expand all nodes | collapse all nodes | view schema
| Expr9631 | Expression_of | Gene | WBGene00008400 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00008400 | ||
| Expression_data | GO_term | GO:0005634 | |
| GO:0005737 | |||
| GO:0043186 | |||
| GO:0000794 | |||
| GO:0070090 | |||
| Subcellular_localization | Finally, all aspects of the localization patterns were absent in each respective mutant background. | ||
| In the mitotic cells of embryos, each factor became enriched in prophase nuclei. As chromosomes condensed, DRH-3, EGO-1, and EKL-1 became enriched along the length of each chromosome, while CSR-1 remained nuclear. All four proteins exhibited robust localization around the metaphase plate. CSR-1 and DRH-3 displayed a pattern similar to cohesins, whereas EKL-1 (and to a lesser degree, EGO-1) appeared to be more closely associated with chromosomes in a pattern similar to kinetochore proteins. In fact, EKL-1 retained a robust association with chromosomes during anaphase, whereas the other RNAi factors became more difficult to detect. Cytoplasmic localization was also detected for each protein. | |||
| The larger CSR-1 isoform was expressed throughout larval development and was also present at low levels in post-mitotic populations lacking a germline. Quantitative real-time RT-PCR analysis of both csr-1 transcripts indicated that their expression recapitulates the protein expression pattern. DRH-3, EGO-1, and CSR-1 colocalize in the germline with PGL-1, a previously characterized component of the germline nuage structures called P granules. EKL-1 was not detected in P granules. | |||
| While many developmentally important factors transiently localize to P granules, DRH-3 and CSR-1 maintained their P granule localization in germ cells throughout the life cycle. As oocytes matured, EGO-1 was lost from the P granules, while DRH-3 and CSR-1 maintained P granule association. In mature oocytes, CSR-1 and EGO-1 both became enriched in nuclei, where CSR-1 was enriched on the diakinetic chromosomes. | |||
| Type | Antibody | ||
| Picture | WBPicture0000009467 | ||
| WBPicture0000009473 | |||
| Reference | WBPaper00035270 | ||
| Antibody_info | WBAntibody00001998 | ||
| WBAntibody00001999 |
