WormBase Tree Display for Expr_pattern: Expr9720

Expr9720 Expression_of: Gene: WBGene00000915
    Reflects_endogenous_expression_of: WBGene00000915
  Expression_data: Anatomy_term: WBbt:0005451 Certain
      WBbt:0005736 Certain
      WBbt:0005792 Certain
      WBbt:0005813 Uncertain
        Remark: Sporadic expression could be observed in body wall muscle cells and vulval muscles.
      WBbt:0005821 Uncertain
        Remark: Sporadic expression could be observed in body wall muscle cells and vulval muscles.
      WBbt:0006751 Partial
        Remark: Fluorescence was observed in single head neurons.
  Type: Reporter_gene
  Pattern: The daf-21 promoter led to GFP expression in the excretory system, pharyngeal muscle cells and intestinal cells. Furthermore, sporadic expression could be observed in body wall muscle cells and vulval muscles. In order to confirm the sporadic expression in muscles, we constructed another reporter plasmid, containing a 2.5 kb genomic sequence upstream of the DAF-21 start codon including its first 4 amino acids fused to YFP.The fluorescent tissues corresponded to those of the stable line and the sporadic expression in body wall muscle cells was recapitulated. Additionally, fluorescence in single head neurons was observed.
  Picture: WBPicture0000012584
  Experiment: Strain: WBStrain00001265
  Reference: WBPaper00040286
  Transgene: WBTransgene00031168