WormBase Tree Display for Expr_pattern: Expr9861

Expr9861 Expression_of: Gene: WBGene00018572
    Reflects_endogenous_expression_of: WBGene00018572
  Expression_data: Life_stage: WBls:0000037
      WBls:0000040
      WBls:0000026
      WBls:0000029
    Anatomy_term: WBbt:0005451 Certain
      WBbt:0005734 Certain
      WBbt:0005753 Certain
  Type: Reporter_gene: [lin-42ap::gfp-pest] transcriptional fusion. The start codon of lin-42a and 3,043 bps of upstream sequence were fused with gfp-pest. --precise ends.
  Pattern: GFP expressed from the lin-42a promoter was robustly expressed in the pharyngeal myoepithelium midway through the L1 stage and continuously thereafter. The lin-42a reporter was also expressed in hyp7 and the lateral seam cells of late L1-stage larvae undergoing the first molt. Expression of the lin-42a reporter in the hypodermis was detected later in the L1 stage than expression of the lin-42b reporter. Fluorescence from the lin-42a reporter also persisted through ecdysis, whereas fluorescence from the lin-42b reporter dissipated during lethargus.
    To fully define the cyclical expression pattern of the lin-42ap::gfp-pest fusion gene, we measured the relative intensity of the associated fluorescence in the hypodermis throughout larval development. The intensity of fluorescence repeatedly increased 2- to 10-fold during the molts and dropped to background levels early in the next life stage.
  Picture: WBPicture0000012605
  Reference: WBPaper00040467
  Transgene: WBTransgene00014753