e723 : limp paralysed or very slow thin Egl; abnormal muscle ultrastructure with large aggregates of thin filaments; recessively suppressed by sup-12. ES3 ME0. NA > 10 (e677 (pka unc-66) e890 : both resemble e723 etc.).
See unc-60.
See also e677, e723, e890, s1310, s1586, s1983, s1986
[C.elegansII] e723 : limp paralysed or very slow, thin, Egl; abnormal muscle ultrastructure with large aggregates of thin filaments; recessively suppressed by sup-12. ES3 ME0. OA>10: e677 (pka unc-66), e890 (both resemble e723), e890, s1310 (induced on eT1), s1586 (500 bp deletion, larval lethal). Also r398, s1307 (both antidystrophic alleles). Cloned: 1.3 and 0.7 kb alternative transcripts encoding 165 aa and 152 aa predicted proteins with 38% identity, sharing only first exon and first methionine; both have similarity to actin depolymerizing proteins (cofilin, destrin). [Waterston et al. 1980; McKim et al. 1994; BC]
unc-60 encodes two, tissue-specific isoforms of actin depolymerizing factor(ADF)/cofilin, actin-binding proteins that regulate actin filament dynamics; one UNC-60 isoform, UNC-60A, primarily binds G-actin, depolymerizes actin filaments, and inhibits actin polymerization; UNC-60A is essential during early embryonic development for polar body extrusion, cytokinesis, and embryonic patterning; UNC-60B, which does not inhibit actin polymerization, binds F- and G-actin and influences the rate of actin polymerization by the UNC-60B:actin ratio; UNC-60B is required for actin filament organization in body wall muscle, locomotion, and for efficient actin filament disassembly by UNC-78, an actin interactin protein 1 (AIP1) ortholog; UNC-60A and UNC-60B are differentially expressed: UNC-60A is found in oocytes, embryos, and the adult gonad and intestine, while UNC-60B is found in body wall muscle, the vulva, and spermatheca; unc-60 transcripts undergo muscle-specific pre-mRNA processing, regulated by the ASD-2 and SUP-12 splicing factors.
Enables actin filament binding activity. Involved in several processes, including actin filament-based process; embryo development; and positive regulation of actin filament polymerization. Located in striated muscle thin filament. Part of ribonucleoprotein complex. Expressed in several structures, including germ line; hermaphrodite gonad; nerve ring; oocyte; and vulva. Human ortholog(s) of this gene implicated in brain compression; gallbladder carcinoma; and nemaline myopathy 7. Is an ortholog of human CFL2 (cofilin 2).
[ Wakarchuck MF] dpy-18(e364)/eT1; unc-60(m35)/eT1 male was crossed to eT1(III;V); sEx9 [pRF4{rol-6(su1006)}+C32E5] hermaphrodite. F1 rollers segregated fertile Dpy non-Uncs among their progeny, indicating rescue of unc-60(m35).