WormBase Tree Display for Gene: WBGene00002482
expand all nodes | collapse all nodes | view schema
WBGene00002482 | Identity | Version | 1 | ||||||
---|---|---|---|---|---|---|---|---|---|
Name | CGC_name | let-253 | Person_evidence | WBPerson533 | |||||
Public_name | let-253 | ||||||||
Species | Caenorhabditis elegans | ||||||||
History | Version_change | 1 | 07 Apr 2004 11:29:28 | WBPerson1971 | Event | Imported | Initial conversion from geneace | ||
Status | Live | ||||||||
Gene_info | Gene_class | let | |||||||
Reference_allele | WBVar00089091 | ||||||||
Allele | WBVar00089091 | Inferred_automatically | From strain object: SP212 | ||||||
WBVar00090465 | |||||||||
Legacy_information | mn181 : larval lethal. NA2 (mn184). | ||||||||
[Labouesse M] F31B8 was injected into a heterozyte ahrboring a new let-253 allele n2142 dpy-10(e128) let-253(n2142) unc-4(e120)/mnC1 together with a dpy-10(+) plasmid provided by J.Kramer; injections with C24G10 and C18D9 were done in the let-253(mn181) unc-4 (e120)/mnC1 background using rol-6(su1006) as the marker. Other injections with subclones of cosmid K06E5 confirmed let-253 not to be under K06E5. | |||||||||
[C.elegansII] mn181 : larval lethal; some embryonic and larval death or very slow growth to Egl adult.Severe constipation; most intestinal nuclei fail to divide in L1. OA2: mn184, n2142. Cloned: cosmid rescue (F31B8). [Sigurdson et al. 1984; SP; ML] | |||||||||
Complementation_data | [Labouesse M] F31B8 (2 F2 recued lines/3 lines); C24G10 (9 F2 rescued lines/13 F2 lines);C18D9 (0 F2 rescued lines/6 F2 lines) | ||||||||
Strain | WBStrain00034155 | ||||||||
Structured_description | Concise_description | let-253 was identified in screens for EMS-induced recessive lethal mutations on chromosome II; let-253 mutations result in low levels of embryonic and larval lethality with survivors exhibiting very slow growth and egg-laying defects upon reaching adulthood; let-253 mutant animals are severely constipated and display a failure of intestinal nuclear divisions in the L1 larval stage. | Paper_evidence | WBPaper00000715 | |||||
WBPaper00014501 | |||||||||
Curator_confirmed | WBPerson1843 | ||||||||
Date_last_updated | 05 Nov 2010 00:00:00 | ||||||||
Map_info | Map | II | Position | 0.50773 | Error | 0.003824 | |||
Well_ordered | |||||||||
Positive | Positive_clone | C24G10 | |||||||
F31B8 | |||||||||
Negative | Negative_clone | C18D9 | |||||||
Mapping_data | Multi_point | 822 | |||||||
3148 | |||||||||
3149 | |||||||||
Pos_neg_data | 1076 | ||||||||
1388 | |||||||||
1470 | |||||||||
1495 | |||||||||
2370 | |||||||||
2373 | |||||||||
Reference | WBPaper00000715 | ||||||||
WBPaper00014501 | |||||||||
WBPaper00029020 | |||||||||
Remark | [Labouesse M] this places let-253 immediately to the right of lin-26 on LGII with the order dpy-10 lin-26 let-253 unc-4 this conclusion contradicts in part the current genetic map which shows the deficiency mnDf97 to complements let-253; data summarized above indicate that mnDf that mnDf97 breaks into C24G10 and that C24G10 complements let-253 both on the left side of C24G10. I have not repeated the cross to determine whether mnDf97 complements let-253 and I guess that my results can still be compatible with the current map. | ||||||||
Method | Gene |