WormBase Tree Display for Interaction: WBInteraction000501555
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| WBInteraction000501555 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00000908 | Interactor_type | Trans_regulator | |
| WBGene00003048 | Interactor_type | Trans_regulated | |||
| Expr_pattern | Expr3448 | ||||
| Variation_interactor | WBVar00241522 | Interactor_type | Trans_regulator | ||
| WBVar00241610 | Interactor_type | Trans_regulator | |||
| Interaction_summary | Wild-type LIT-1::GFP expression was greatly diminished in daf-12 mutant worms (rh61rh412). Pharyngeal expression was abrogated in constructs bearing the 4.2 m11m31 mutations. DAF-12 probably binds to a DR5 element within 4.2 and activates LIT-1 gene expression in the C. elegans pharynx. Strikingly, the LIT-1::GFP construct with the 4.2 m11m31 mutant response element produced increased GFP expression in seam and vulva cells at larval stage 4 (L4) in wild-type animals, compared with the wild-type 4.2 construct. Elevated expression was also observed when the wild-type LIT-1::GFP construct was microinjected into the daf-12 (rh61rh412) mutant strain. | ||||
| Detection_method | Reporter_gene | [lit-1::gfp] translational fusion. | |||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Negative_regulate | Anatomy_term | WBbt:0005753 | ||
| WBbt:0006748 | |||||
| Paper | WBPaper00024491 |
