WormBase Tree Display for Interaction: WBInteraction000525154

WBInteraction000525154 Interaction_type: Regulatory: Change_of_expression_level
  Interactor: Interactor_overlapping_gene: WBGene00002993 Interactor_type: Trans_regulator
          Trans_regulated
        Expr_pattern: Expr11602
        Transgene: WBTransgene00005172
        Construct: WBCnstr00005149
      WBGene00198855 Interactor_type: Trans_regulator
      WBGene00199631 Interactor_type: Trans_regulator
      WBGene00197123 Interactor_type: Trans_regulator
      WBGene00019128 Interactor_type: Trans_regulator
    Variation_interactor: WBVar00143592 Interactor_type: Trans_regulator
  Interaction_summary: In a wild-type background, basal levels of GFP expression from the plin-4:GFP transgene are first detectable in the early L2 stage in the seam cells of the worm. Interestingly, 2 distinct bursts of augmented GFP expression are observed, initiated in the early L2 and early L4 stages of development. Comparatively, we detected significantly fewer plin-4:GFP-positive seam cells in both the L2 and L4 in the lin-4 mutants, where no endogenous lin-4 is produced. Quantitative PCR (qPCR) analysis of total GFP mRNA from mixed-stage wild-type and mutant animals confirmed that this observation in the seam cells is also true as a general trend for GFP expression when assayed from throughout the animal: 3 times more GFP mRNA is transcribed from the reporter construct in the wild-type background than in the lin-4 mutant background. Thus, lin-4 is required to promote activity from its own promoter.
  Detection_method: Construct
    Reporter_gene: [lin-4::gfp]
    Transgene
    RT_PCR
  Regulation_level: Transcriptional
  Regulation_result: Positive_regulate: Anatomy_term: WBbt:0005753
  Paper: WBPaper00044681