WormBase Tree Display for Interaction: WBInteraction000554745
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WBInteraction000554745 | Interaction_type | Physical | ProteinProtein | ||
---|---|---|---|---|---|
Interactor | Interactor_overlapping_gene | WBGene00000912 | Interactor_type | Bait | |
WBGene00010923 | Interactor_type | Target | |||
Interaction_summary | RLE-1 was detected by coimmunoprecipitation and western blotting when it was coexpressed with DAF-16 but not in the control lane of RLE-1 alone. | ||||
RLE-1 was detected in the anti-DAF-16 immunoprecipitate when lysates of wild-type worms but not of rle-1 mutants were used. | |||||
RLE-1 colocalizes with DAF-16 in C. elegans as demonstrated by confocal microscopy. | |||||
Deletion of the C terminus of RLE-1 completely abolished its interaction with DAF-16, indicating the RLE-1 C terminus is required for the interaction of RLE-1 with DAF-16. | |||||
Detection_method | Affinity_capture_Western | ||||
Throughput | Low_throughput | ||||
WBProcess | WBbiopr:00000001 | ||||
DB_info | Database | BioGRID | Interaction ID | 555975 | |
Paper | WBPaper00029081 |