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WBPicture0000007368 | Description | (B) The expression reporter transgenes magi-1L::gfp and magi-1::rfp. Diagram labeling is as described for Figure 1. (C) The magi-1L:::gfp reporter transgene reflects the expression of the long isoform. Expression of MAGI-1L::GFP was detected in the pharynx, head neurons, ventral cord, and vulva of animals mosaic for the extrachromosomal array. (D) The magi-1::rfp reporter transgene reflects the expression of both isoforms. Neurons in the head are shown in animals that coexpress GLR-1::GFP. Expression of the reporter in the pharynx is out of the plane of focus. (E) Expression of MAGI-1L::RFP was also detected in (from left to right) the pharynx, intestine, vulval epithelia (L4 stage), and vulval muscle (adult stage) of animals mosaic for the extrachromosomal array. For pictures of the pharynx, it should be noted that neurons expressing MAGI-1 are out of the plane of focus. Unlike the MAGI-1L:::GFP reporter, which contains only the PDZ0 domain and is therefore unlocalized inside cells, the full length MAGI-1::RFP construct is localized to cellular junctions and punctate structures within cells. | |||
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Name | journal.pone.0004613.s002_BCDE.jpg | ||||
Crop | Cropped_from | WBPicture0000007367 | |||
Depict | Expr_pattern | Expr8581 | |||
Anatomy | WBbt:0003681 | ||||
WBbt:0005772 | |||||
WBbt:0005821 | |||||
WBbt:0005829 | |||||
WBbt:0006748 | |||||
WBbt:0006751 | |||||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
Publication_year | 2009 | ||||
Article_URL | DOI | id | 10.1371/journal.pone.0004613 | ||
Journal_URL | PLoSOne | ||||
Publisher_URL | PLoS | ||||
Reference | WBPaper00032939 |