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| WBPicture0000007467 | Description | Figure 8 Characterization of the three candidate genes identified from the functional RNAi screens. (A) Heme-dependent expression. qRT-PCR was performed in triplicate for hrg-4, mrp-5, and F22B5.4 at the indicated heme concentrations, and the fold change (mean +/- SEM) is reported for each gene at each concentration of heme. (B) Accumulation of ZnMP in worms depleted of candidate genes by RNAi. Synchronized wild-type strain worms in the L1 stage were transferred from mCeHR-2 medium containing 2 uM heme to plates seeded with a lawn of bacteria producing dsRNA against vector, hrg-4, mrp-5, or F22B5.4 and allowed to develop for 72 h to the late L4 stage. At this point, worms were incubated in mCeHR-2 medium containing 5 uM ZnMP overnight. Images of a region of worm intestine were captured using a Leica DMIRE2 microscope equipped with a Rhodamine filter and a CCD camera. (C) Expression of hrg-4 and mrp-5 GFP transcriptional reporters. Transgenic worms expressing GFP under the control of 3 kb of the hrg-4 (bottom) or mrp-5 (top) putative promoters. Transgenic worms were grown in mCeHR-2 medium containing 4 uM heme for one generation. Representative images of worms were obtained with a Leica DMIRE2 microscope using a CCD camera. | |||
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| Name | journal.pgen.1001044.g008_C2.jpg | ||||
| Crop | Cropped_from | WBPicture0000007465 | |||
| Depict | Expr_pattern | Expr9100 | |||
| Anatomy | WBbt:0005772 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2010 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1001044 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00036738 |
