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WBPicture0000007527 | Description | Figure 3. DPY-22::GFP is expressed in the nuclei of vulval cells, and is mislocalized by the dpy-22(sy665) mutation. Left-hand images are Nomarski, and right-hand images are fluorescence. Scale bars: 20 μm. Arrows indicate some of the vulval cells and arrowheads indicate the anchor cells. (A-F) dpy-22(sy622) hermaphrodites rescued with a dpy-22::gfp transgene that encodes a fusion of full-length wild-type DPY-22 to GFP. (A,B) Two-cell stage at the onset of vulval fate specification. (C,D) Four-cell stage of vulval development. (E,F) Eight-cell stage of vulval development. (G,H) dpy-22(sy622) hermaphrodites rescued with a dpy-22 2548::gfp transgene, which encodes a fusion of the first 2548 amino acids of DPY-22 to GFP. (I,J) pha-1(e2123ts)-rescued animals carrying a dpy-22 2141::gfp transgene, which encodes a fusion of the first 2141 amino acids of DPY-22 to GFP. Wild-type DPY-22 and DPY-22 truncated after amino acid 2548 direct GFP to the nucleus, but not DPY-22 truncated after amino acid 2141. | |||
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Name | F3.large.jpg | ||||
Crop | Crop_picture | WBPicture0000007528 | |||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2003 | ||||
Article_URL | DOI | id | 10.1242/dev.00189 | ||
Journal_URL | Development | ||||
Publisher_URL | TheCompanyofBiologists | ||||
Reference | WBPaper00005632 |