WormBase Tree Display for Picture: WBPicture0000007642

WBPicture0000007642 Description: Figure 3. Localization of exported MSP and specificity of release. (A) Localization of MSP at the surface of the -1 oocyte. Single confocal sections at the indicated level of a 3D data stack through a mated female gonad stained for MSP (red) and RME-2 yolk receptor (green). No spermatozoa were seen in the indicated region (line) of the spermatheca (sp), thus the staining observed is extracellular to spermatozoa. A projection of the entire stack is presented as Movie 3 in the supplementary material. (B) Single angle views of a 3D reconstruction of the data stack represented in A. The image is cut to show surface and interior views of the -1 oocyte, at the indicated angles. Overlap between the MSP (red) and RME-2 (green) signals is yellow. Note the oocyte surface is slightly compressed where it abuts the spermatheca. The entire reconstruction is presented as Movie 4 in the supplementary material. (C) Intact and viable spermatozoa release MSP, as shown by a mated female stained for DNA (blue), MitoTracker (red) and MSP (green). Note, the MitoTracker staining is limited to the spermatozoa (s), but the MSP staining extends at least 50 μm from the most distal spermatozoa (arrowhead). (D) MSP localizes to extracellular puncta and apparent buds at the spermatozoa surface. Projections of confocal 3D data stacks from mated females stained in wholemount for MSP (red) and MSD proteins (green), with overlap in yellow. Images are superimposed on the DIC channel, showing spermatozoa (s) in the uterus. Puncta (arrows) and surface blebs (arrowheads) contain MSP, but not MSD proteins. A 3D projection of similar data is presented in Movie 5 in the supplementary material. (E) Budding generates MSP puncta. Single angle views of a 3D reconstruction of MSP (red) and MSD (green) staining, with overlap in yellow. The image is cut to show interior and surface views of the spermatozoa. Apparent sites of budding contain MSP but not MSD. The entire reconstruction is presented as Movie 6 in the supplementary material. Scale bars: 20 μm.
  Name: F3.large.jpg
  Depict: Expr_pattern: Expr3417
    Anatomy: WBbt:0005178
      WBbt:0005319
      WBbt:0006760
      WBbt:0006794
      WBbt:0006797
      WBbt:0006798
    Cellular_component: GO:0005938
      GO:0009986
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2005
    Article_URL: DOI id 10.1242/dev.01916
    Journal_URL: Development
    Publisher_URL: TheCompanyofBiologists
  Reference: WBPaper00026617