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WBPicture0000007816Description(A,B) In situ hybridization using a mig-6L-specific probe showing DTC (d) specific expression (A), and using a probe common for mig-6S and mig-6L showing expression in the body wall muscles and other unidentified cells (B). (C-E) From L2 to adult stages, a mig-6 promoter::gfp transcriptional reporter construct is expressed in DTCs (d) (C), in body wall muscles (m), in the CAN neuron (can) (D), in the head mesodermal cell (hmc), in GLR cells (glr) and in coelomocytes (cc) (E). (F) Anti-MIG-6 antibody staining of late embryos (released from their shells) shows MIG-6 protein is expressed by body wall muscle primordium (green) in wild types. (H) In early larval stages, anti-MIG-6 antibody stains basement membranes of the gonad primordium (g), pharynx (ph) and intestine (i). This staining persists throughout larval and adult stages. (I,J) Anti-MIG-6 antibody stains basement membrane of the gonad and migrating DTCs (d) of L3 (I) and L4 (J) stages. Enlargement in I shows staining on the DTC. Vulvae are indicated by white triangles. Asterisks indicate posterior. Dorsal is upwards in all panels. Scale bar: 20 μm.
NameF4.large_A.jpg
CropCropped_fromWBPicture0000007815
Remarkexpressed in the body wall muscle primordium
DepictExpr_patternExpr8562
Expr8563
Expr8564
AnatomyWBbt:0004697
WBbt:0005176
WBbt:0005751
WBbt:0005813
WBbt:0006827
WBbt:0006865
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2009
Article_URLDOIid10.1242/dev.028472
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00033006