WormBase Tree Display for Picture: WBPicture0000007915
expand all nodes | collapse all nodes | view schema
| WBPicture0000007915 | Description | Figure 3. Expression of ceh-10. (A-E) The expression pattern of pS310 ceh-10/lacZ trans-genes (Fire et al., 1990) in embryos and larvae (scale bar = 10 µm). Anterior is left. (A) The premorphogenesis embryo shows the first detectable β-galactosidase staining, a singlenucleus at the extreme anterior end of the embryo. This embryo has approximately 350 cells (determined by comparing nuclear patterns revealed by DAPI co-staining to line drawings of live embryos; Sulston et al., 1983). (B) Two cells stain in late proliferation stage embryos. (C) The comma stage embryo has six nuclei staining at the anterior end of the embryo. As the embryo lengthens two more nuclei appear in the staining pattern; (D) the 1.5-fold embryo has six anterior staining nuclei and two staining nuclei (arrow) near the excretory pore (only one of these two nuclei is seen in this plane of focus). The position of these two nuclei is consistent with their being AIY cells (see text). (E) An L1 larva is shown. The identity of the stained nuclei in L1 stage worms (see text) was determined by Dr C. Bargmann (UCSF, personal communication; cells identified as occasionally staining for β-galactosidase activity include sensoryneuron support cells CEPshDL and OLQso). Two to four cells stain in the tail anterior to the pre-anal ganglion, but their identity has not yet been determined. (F) An example of process staining of the AIY cell is shown; the arrowhead marks the position of the ventral side of the worm at the nerve ring. (G,H) Transgenic worms (pInt 1) costained with the DNA stain DAPI and immunostained for beta-galactosidase protein. (G) A confocal image (0.5 µm slice thickness)of DAPI stained nuclei in the right ventral ganglion (see Fig. 3 in White et al., 1986). (H) Rhodamine fluorescence detected in the same image plane. Outlines of the four nuclei labelled in G are superimposed on the rhodamine image in H, identifying AIYR as the ceh-10-expressing cell. | |
|---|---|---|---|
| Name | FigF.jpg | ||
| Depict | Expr_pattern | Expr1584 | |
| Anatomy | WBbt:0003961 | ||
| WBbt:0003963 | |||
| WBbt:0004423 | |||
| WBbt:0004424 | |||
| WBbt:0004425 | |||
| WBbt:0004426 | |||
| WBbt:0004929 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 1995 | ||
| Journal_URL | Development | ||
| Publisher_URL | TheCompanyofBiologists | ||
| Reference | WBPaper00002186 |
