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WBPicture0000007962DescriptionFigure 5. UNC-37 is nuclear-localized and ubiquitously expressed. (A) Immunoblot showing that anti-UNC-37 stains the GST2 fusion protein in bacterial extracts and a single approx. 70x10<sup>3</sup> M<sub>r</sub> band in C. elegans wild-type(N2) homogenate. (B) Anti-UNC-37 stained and (C) DIC image of wild-type hermaphrodite gonad. Enlarged, brightly stained oocyte nuclei occupy the proximal arm of the gonad. Arrows denote sheath cell nuclei. (D) Distal arm of wild-type hermaphrodite gonad stained with anti-UNC-37. Asterisk marks distal tip cell. (E) Wild-type embryo (seven cell nuclei) and (F) L1 larva stained with anti-UNC-37. (G) Posterior region of late L1/early L2 larva stained with DAPI (blue) to mark all cell nuclei and with anti-&beta;-galactosidase (red) to identify lacZ-positive motor neurons in the unc4-lacZ transgenic strain, wdIs1. unc-4-lacZ is expressed in all DA and VA motor neurons in the ventral nerve cord; however, VA8, VA11, and VA12 do not express lacZ in this animal. lacZ-stained cells in the preanal ganglion (PAG) are DA8 and DA9. (H) Same animal as in G, stained with anti-UNC-37 (green). Cell nuclei of DA and VA motor neurons are marked with white lines. Horizontal scale bars, 20 &micro;m.
NameFigure5.jpg
DepictExpr_patternExpr329
AnatomyWBbt:0005175
Cellular_componentGO:0005634
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1997
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00002798