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| WBPicture0000008103 | Description | Figure 9. Detection of SEC-1 mRNA and protein in situ. For A and B, C. elegans were fixed, permeabilized, and then hybridized with digoxigenin-labeled, SEC-1 antisense DNA as described previously (55). RNA-DNA complexes were visualized by incubating the specimens serially with antidigoxigenin IgGs coupled to alkaline phosphatase and a chromogenic substrate. Alkaline phosphatase catalyzes the synthesis of an insoluble reaction product (shown as a black precipitate) in cells expressing SEC-1 mRNA. A, immature embryos (arrows) stain intensely for SEC-1 mRNA. Embryos that have undergone morphological transitions that result in the development of the classical C. elegans body shape (arrowheads) have low or undetectable levels of SEC-1 mRNA. B, L4 larvae and adult (not shown) C. elegans selectively express SEC-1 mRNA in developing oocytes in the gonad. Specimens in A and B were photographed using Nomarski interference optics with a Zeiss Axioscop microscope as described previously (40). C, C. elegans embryos were fixed, permeabilized, and incubated with antibodies directed against SEC-1 as described previously (45). Subsequently, samples were incubated with fluorescein isothiocyanate-coupled goat IgGs directed against rabbit immunoglobulins (45). Fluorescence signals corresponding to SEC-1-IgG complexes were obtained with a Bio-Rad MRC 600 laser scanning confocal microscope as described previously (45). Immature embryos (arrows) contain substantial levels of SEC-1 protein, whereas nematodes undergoing morphogenesis stain weakly (arrowheads) or remain unstained (10 additional mature embryos, which are observed in this field by phase microscopy, are not visualized by immunofluorescence). | |||
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| Name | F8.large.jpg | ||||
| Depict | Expr_pattern | Expr1607 | |||
| Expr1608 | |||||
| Anatomy | WBbt:0005175 | ||||
| WBbt:0007028 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1996 | ||||
| Article_URL | DOI | id | 10.1074/jbc.271.47.30158 | ||
| Journal_URL | TheJournalofBiologicalChemistry | ||||
| Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
| Reference | WBPaper00002615 |
