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| WBPicture0000008300 | Description | Figure 7. Expression and localization of DKF-1 in C. elegans. Lines of transgenic C. elegans that express full-length DKF-1::GFP under control of the dkf-1 gene promoter/enhancer were created as indicated under Experimental Procedures. A, mixed populations of WT and transgenic animals were extracted with SDS-PAGE loading buffer. Proteins were separated by denaturing electrophoresis. Expression of DKF-1 fusion protein was assessed by Western immunoblot analysis as described under Experimental Procedures. Endogenous DKF-1 (81 kDa) as well as DKF-1 GFP fusion protein (107 kDa) are detected by anti-DKF-1 IgGs. DKF-1 GFP is not present in WT animals. B-D, cells enriched in DKF-1 protein were identified by fluorescence microscopy (x600 magnification). B, DKF-1 is expressed prominently in cells associated with the posterior bulb of the pharynx. Nomarski interference images of worms are labeled with N (BN, CN, DN). DKF-1 GFP accumulates in neurons (arrows) in adult animals. Anterior (a) and posterior (p) bulbs of the pharynx are shown in panel BN. B2, cell-specific DKF-1 GFP accumulation is also evident in a lateral view of the posterior pharyngeal bulb (arrow). C, DKF-1 is abundantly expressed in cells positioned near the anterior pharyngeal bulb. Two neurons that selectively express the reporter-dkf-1 gene are marked with arrows. D, fluorescence signals corresponding to DKF-1 GFP emanate from cell bodies, axons, and dendrites of a subset of tail neurons (arrows). | |||
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| Name | F7.large.jpg | ||||
| Depict | Expr_pattern | Expr7808 | |||
| Anatomy | WBbt:0003679 | ||||
| WBbt:0003681 | |||||
| WBbt:0006759 | |||||
| Cellular_component | GO:0030424 | ||||
| GO:0030425 | |||||
| GO:0043025 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2006 | ||||
| Article_URL | DOI | id | 10.1074/jbc.M511899200 | ||
| Journal_URL | TheJournalofBiologicalChemistry | ||||
| Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
| Reference | WBPaper00027322 |
