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WBPicture0000008303 | Description | Figure 2. Temporal pattern of cpi-2a gene expression as determined by real time PCR. The levels of the cpi-2a transcript in each stage of development were measured and compared with the previously determined transcript levels of C. elegans Ce-cpl-1 and cpz-1. The graph shows the ratio +/- S.D. between cpi-2a or cpl-1 (A) and cpi-2a or cpz-1 (B) levels and those of the constitutively expressed control gene ama-1 (y axis). The ama-1 gene was used as an internal control transcript to allow the relative quantification of cpi-2a, cpl-1, and cpz-1 expression in distinct developmental stages (x axis). The RNA from mixed stage embryos and from synchronized larval and adult populations collected at 2-h intervals was isolated for the preparation of stage-specific cDNA. Stage-specific molting within the life cycle is indicated by a vertical arrow. Each experimental point was repeated at least twice. | |||
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Name | F2.large.jpg | ||||
Depict | Expr_pattern | Expr7868 | |||
Expr7869 | |||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2006 | ||||
Article_URL | DOI | id | 10.1074/jbc.M600254200 | ||
Journal_URL | TheJournalofBiologicalChemistry | ||||
Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
Reference | WBPaper00027761 |